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Modafinil ameliorated pancreatic injury and inflammation through upregulating SNIP1

Jianwu Wu1, Yijie Lu1, Ancheng Qin1

  • 1Department of Hepatobiliary and Pancreatic Surgery, Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Suzhou, Jiangsu, China.

General Physiology and Biophysics
|September 9, 2020
PubMed

Insights

Modafinil demonstrates significant therapeutic effects against acute pancreatitis (AP) by reducing inflammation, oxidative stress, and apoptosis. This action is mediated through the regulation of SNIP1 levels, offering a potential new treatment for AP.

Area of Science:

  • Gastroenterology
  • Pharmacology
  • Molecular Biology

Background:

  • Acute pancreatitis (AP) is a severe inflammatory condition of the pancreas.
  • Modafinil exhibits known anti-inflammatory and anti-oxidative properties.
  • The therapeutic potential of modafinil in AP has not been previously investigated.

Purpose of the Study:

  • To investigate the therapeutic effects of modafinil on acute pancreatitis in vivo and in vitro.
  • To elucidate the underlying molecular mechanisms of modafinil's action in AP.
  • To assess modafinil's impact on pancreatic inflammation, oxidative stress, and apoptosis.

Main Methods:

  • An in vivo rat model of AP was established using sodium taurocholate injection.
  • In vitro AP models were created using AR42J cells stimulated with cerulein and lipopolysaccharide.
  • Assays included HE staining, serum amylase/lipase, ELISA, MDA/SOD kits, TUNEL staining, Western blotting, qPCR, and flow cytometry.
  • SNIP1 gene silencing was employed to explore modafinil's mechanism.

Main Results:

  • Modafinil treatment significantly alleviated AP severity, reducing inflammation, oxidative stress, and apoptosis in the rat model.
  • In vitro studies confirmed modafinil's protective effects against AP-induced cellular damage.
  • Silencing SNIP1 reversed the beneficial effects of modafinil, indicating SNIP1's crucial role.

Conclusions:

  • Modafinil possesses significant curative and therapeutic effects for acute pancreatitis.
  • Modafinil exerts its protective effects by regulating SNIP1 expression.
  • These findings suggest modafinil as a potential therapeutic agent for managing AP.

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