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Comprehensive Identification and Characterization of Long Non-coding RNAs Associated With Rice Black-Streaked Dwarf
Jian-Hua Zhang1, Yan Dong1,2, Wei Wu1
1Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
Frontiers in Physiology
|September 9, 2020
Summary
Long non-coding RNAs (lncRNAs) regulate Rice black-streaked dwarf virus (RBSDV) transmission in the planthopper vector Laodelphax striatellus. Targeting lncRNAs or their associated protease inhibitor (PI) impacts RBSDV accumulation, revealing novel regulatory roles in insect-borne viral diseases.
Area of Science:
- Molecular Biology
- Virology
- Entomology
Background:
- Long non-coding RNAs (lncRNAs) are key regulators of gene expression.
- Their role in insect-mediated virus transmission remains largely unexplored.
- Understanding lncRNA function is crucial for managing insect-borne viral diseases.
Purpose of the Study:
- To identify and characterize differentially expressed lncRNAs in the midgut of Laodelphax striatellus upon Rice black-streaked dwarf virus (RBSDV) infection.
- To investigate the functional roles of these lncRNAs and their target genes in RBSDV transmission.
- To explore potential regulatory mechanisms involving lncRNAs in insect-virus interactions.
Main Methods:
- RNA sequencing (RNA-Seq) to identify differentially expressed lncRNAs.
- RT-qPCR for expression validation of lncRNAs and target genes.
- KEGG pathway analysis to determine enriched biological processes.
- RNA interference (RNAi) to assess the functional impact of lncRNA and target gene knockdown.
Main Results:
- 13,927 lncRNAs were identified, with 176 showing differential expression in response to RBSDV infection.
- Differentially expressed lncRNAs targeted 168 trans-regulatory genes involved in purine metabolism, amino acid degradation, and fatty acid elongation.
- Knockdown of lncRNA MSTRG15394 or its target protease inhibitor (PI) significantly increased RBSDV accumulation in L. striatellus midgut.
Conclusions:
- lncRNAs play a significant role in regulating RBSDV infection in the insect vector Laodelphax striatellus.
- The lncRNA MSTRG15394 and its target PI are involved in controlling RBSDV accumulation.
- These findings offer novel insights into the molecular mechanisms underlying insect-mediated virus transmission and potential targets for disease control.
