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Delta-like Factor 1 as a Possible Therapeutic Target for Sarcomas

Han-Soo Kim1,2, Sun Hee Ahn1, Ha Jeong Kim1

  • 1Department of Orthopedic Surgery, Seoul National University Hospital, Seoul, Korea.

Clinics in Orthopedic Surgery
|September 9, 2020
PubMed
Abstract

Insights

The delta-like factor 1 (DLK1) gene promotes sarcoma growth and invasion. Inhibiting DLK1 showed anti-cancer effects, suggesting DLK1 as a potential therapeutic target for sarcoma treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Cancer stem cells (CSCs) possess self-renewal and tumorigenic capabilities.
  • Sarcomas are a diverse group of cancers originating from connective tissues.

Purpose of the Study:

  • To investigate the role of delta-like factor 1 (DLK1) in sarcoma development and progression.
  • To explore DLK1 as a potential therapeutic target in sarcoma.

Main Methods:

  • Examined DLK1 mRNA expression in 13 sarcoma cell lines.
  • Isolated and analyzed CSCs using fluorescence-activated cell sorting (FACS) and sphere-forming assays.
  • Assessed the impact of DLK1 inhibition via short interfering RNA (siRNA) on sarcoma cell proliferation and invasion.

Main Results:

  • High DLK1 mRNA expression was detected across all sarcoma cell lines.
  • CSCs isolated from DLK1-overexpressing sarcomas showed lower DLK1 expression than wild-type cells.
  • DLK1 inhibition suppressed proliferation and invasion in multiple sarcoma cell lines, including CSCs, and regulated liposarcoma invasiveness.

Conclusions:

  • The DLK1 gene drives tumorigenesis and contributes to the invasive mechanisms in various sarcomas.
  • DLK1 represents a promising therapeutic target for sarcoma treatment.
Keywords:
CD133Cancer stem cellDLK1SarcomaTumorigenesis

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