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Related Concept Videos

Appendicitis-I: Introduction01:22

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Related Experiment Video

Updated: Dec 9, 2025

Murine Appendectomy Model of Chronic Colitis Associated Colorectal Cancer by Precise Localization of Caecal Patch
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Enterochromaffin Cells and Mast Cells in Acute Appendicitis.

Bhavya P Mohan1, K P Aravindan2

  • 1Department of Pathology, Government Medical College, Thrissur, Kerala, India.

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|September 9, 2020
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Summary

Degranulated mast cells, not enterochromaffin cells, are the likely source of increased serotonin in acute appendicitis. This finding impacts understanding of appendicitis pathophysiology.

Keywords:
acute appendicitisallergyenterochromaffin cellsmast cellsserotonin

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Area of Science:

  • Gastroenterology and Pathology
  • Cell Biology and Inflammation

Background:

  • Serotonin levels are elevated in acute appendicitis.
  • The precise source of this serotonin increase remains unclear.
  • Enterochromaffin (EC) cells and mast cells are potential contributors.

Purpose of the Study:

  • To compare the distribution and density of EC cells and mast cells in normal versus appendicitis appendices.
  • To investigate the degranulation status of mast cells in both conditions.
  • To identify the primary source of elevated serotonin in acute appendicitis.

Main Methods:

  • Histological analysis of appendix sections from 15 acute appendicitis and 10 normal cases.
  • Immunohistochemistry for chromogranin (EC cells) and CD-117 (mast cells).
  • Quantification of EC cells and mast cells across different tissue layers, assessing mast cell degranulation.

Main Results:

  • No significant difference in epithelial or extraepithelial EC cell counts between normal and appendicitis appendices.
  • A substantial increase in degranulated mast cells was observed in acute appendicitis compared to normal appendices.
  • Mast cell degranulation appears to be a key feature of acute appendicitis.

Conclusions:

  • Degranulated mast cells are the probable main source of elevated serotonin in acute appendicitis.
  • Enterochromaffin cells do not appear to be the primary driver of increased serotonin.
  • This study reframes the understanding of serotonin's role in appendicitis pathogenesis.