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Updated: Dec 9, 2025
Anti-Nuclear Antibody Screening Using HEp-2 Cells
Published on: June 23, 2014
A Hospital-Based Insight into the Antinuclear Antibody Patterns in Autoimmune Disorders
Prishni Gupta1, Ritu Priya1, Rachita Nanda1
1Department of Biochemistry, All India Institute of Medical Sciences, Raipur, Chhattisgarh, India.
Antinuclear antibody (ANA) testing is crucial for diagnosing autoimmune disorders. In central India, a significant 33% of tested patients showed positive ANA results, predominantly females, highlighting regional autoimmunity prevalence.
Area of Science:
- Immunology
- Clinical Chemistry
Background:
- Rising incidence of autoimmune disorders in India.
- Autoimmune diseases are characterized by antinuclear antibodies (ANA).
- ANA presence in serum is key evidence for autoimmune conditions.
Purpose of the Study:
- Determine ANA antibody patterns.
- Assess the clinical significance of ANA.
- Investigate ANA in a central Indian tertiary care hospital.
Main Methods:
- Retrospective cross-sectional study.
- Analyzed 538 ANA test reports via indirect immunofluorescence assay.
- Statistical analysis included t-test, Chi-square, and Fisher exact tests (p<0.05).
Main Results:
- 33% of 538 patients tested positive for ANA.
- Positive ANA cases were 74% female, mostly young adults.
- The most frequent pattern was nuclear, with a nuclear speckled subpattern.
Conclusions:
- High ANA positivity suggests increased autoimmunity in the region.
- ANA testing is valuable for diagnosing suspected autoimmune disorders.
- Supports ANA's role as diagnostic evidence in central India.
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