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3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer
Published on: September 13, 2018
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Phospholipase C-ε regulates bladder cancer cells via ATM/EXO1
Jiaxin Fan1, Yan Zhao1,2, Hongling Yuan1
1Key Laboratory of Diagnostics Medicine Designated by The Ministry of Education, Chongqing Medical University Chongqing, China.
American Journal of Cancer Research
|September 9, 2020
Summary
Phospholipase C epsilon (PLCε) promotes DNA repair in bladder cancer (BCa) by regulating ATM/EXO1 signaling. MicroRNA-145 counteracts PLCε, enhancing cisplatin sensitivity in BCa cells.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Bladder cancer (BCa) involves complex molecular mechanisms.
- Understanding DNA repair pathways is crucial for BCa treatment.
- Phospholipase C epsilon (PLCε) role in BCa is not well-defined.
Purpose of the Study:
- To elucidate the molecular mechanisms of phospholipase C epsilon (PLCε) in bladder cancer (BCa).
- To investigate the role of PLCε in DNA repair pathways within BCa.
- To identify potential therapeutic targets for BCa treatment.
Main Methods:
- Whole human genome microarray analysis.
- Gene Ontology and KEGG pathway analysis.
- Quantitative gene expression and protein level analysis.
- In vitro studies involving microRNA manipulation.
Main Results:
- Differentially expressed genes in BCa were enriched in DNA repair pathways.
- PLCε, EXO1, and ATM gene expression were significantly upregulated in BCa tissues.
- PLCε expression positively correlated with EXO1 protein levels in BCa samples.
- PLCε facilitates DNA repair in BCa via ATM/EXO1 signaling.
- MicroRNA-145 acts as an antagonist to PLCε by targeting its mRNA.
- Overexpression of microRNA-145 enhanced cisplatin sensitivity in BCa cells.
Conclusions:
- PLCε plays a significant role in DNA repair pathways in bladder cancer.
- The PLCε/ATM/EXO1 signaling axis is a key regulator of DNA repair in BCa.
- MicroRNA-145 represents a potential therapeutic strategy to enhance BCa treatment efficacy, particularly with cisplatin.
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