Related Experiment Video

Updated: Dec 9, 2025

3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer
09:24

3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer

Published on: September 13, 2018

9.4K

Phospholipase C-ε regulates bladder cancer cells via ATM/EXO1

Jiaxin Fan1, Yan Zhao1,2, Hongling Yuan1

  • 1Key Laboratory of Diagnostics Medicine Designated by The Ministry of Education, Chongqing Medical University Chongqing, China.

American Journal of Cancer Research
|September 9, 2020
PubMed

Insights

Phospholipase C epsilon (PLCε) promotes DNA repair in bladder cancer (BCa) by regulating ATM/EXO1 signaling. MicroRNA-145 counteracts PLCε, enhancing cisplatin sensitivity in BCa cells.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Bladder cancer (BCa) involves complex molecular mechanisms.
  • Understanding DNA repair pathways is crucial for BCa treatment.
  • Phospholipase C epsilon (PLCε) role in BCa is not well-defined.

Purpose of the Study:

  • To elucidate the molecular mechanisms of phospholipase C epsilon (PLCε) in bladder cancer (BCa).
  • To investigate the role of PLCε in DNA repair pathways within BCa.
  • To identify potential therapeutic targets for BCa treatment.

Main Methods:

  • Whole human genome microarray analysis.
  • Gene Ontology and KEGG pathway analysis.
  • Quantitative gene expression and protein level analysis.
  • In vitro studies involving microRNA manipulation.

Main Results:

  • Differentially expressed genes in BCa were enriched in DNA repair pathways.
  • PLCε, EXO1, and ATM gene expression were significantly upregulated in BCa tissues.
  • PLCε expression positively correlated with EXO1 protein levels in BCa samples.
  • PLCε facilitates DNA repair in BCa via ATM/EXO1 signaling.
  • MicroRNA-145 acts as an antagonist to PLCε by targeting its mRNA.
  • Overexpression of microRNA-145 enhanced cisplatin sensitivity in BCa cells.

Conclusions:

  • PLCε plays a significant role in DNA repair pathways in bladder cancer.
  • The PLCε/ATM/EXO1 signaling axis is a key regulator of DNA repair in BCa.
  • MicroRNA-145 represents a potential therapeutic strategy to enhance BCa treatment efficacy, particularly with cisplatin.
Keywords:
ATM serine/threonine kinasePhospholipase C epsilonbladder cancerexonuclease 1microRNA-145

More Related Videos

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay13:10

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Published on: June 9, 2017

10.4K
Author Spotlight: A Selective Luciferase-Based Assay for Monitoring ATG4B 27 Activity in Cells09:51

Author Spotlight: A Selective Luciferase-Based Assay for Monitoring ATG4B 27 Activity in Cells

Published on: June 30, 2023

1.3K

Related Concept Videos

Cancer Cell Migration through Invadopodia01:35

Cancer Cell Migration through Invadopodia

Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
3.0K
Cell Motility through Blebbing01:16

Cell Motility through Blebbing

Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
2.3K
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
3.3K
Transducer Mechanism: Enzyme-Linked Receptors01:27

Transducer Mechanism: Enzyme-Linked Receptors

Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
3.6K
Membrane Asymmetry Regulating Transporters01:19

Membrane Asymmetry Regulating Transporters

Enzymes like flippase, floppase, and scramblase transfer phospholipids from one layer to another in the membrane, thereby affecting membrane asymmetry.
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
6.7K
Amplifying Signals via Enzymatic Cascade01:22

Amplifying Signals via Enzymatic Cascade

When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...
16.4K