Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microsomal receptor for steroid hormones: functional implications for nuclear activity.

T G Muldoon1, G H Watson, A C Evans

  • 1Department of Physiology and Endocrinology, Medical College of Georgia, Augusta 30912.

Journal of Steroid Biochemistry
|January 1, 1988
PubMed
Summary

Researchers identified high-affinity microsomal binding sites for estrogen and androgen in rat tissues. These sites may regulate hormone access to the nucleus and influence cellular processes, distinct from known cytosolic receptors.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Crystallization of ApoA1 and ApoE4 nanolipoprotein particles and initial XFEL-based structural studies.

Crystals·2022
Same author

A voxel-wise assessment of growth differences in infants developing autism spectrum disorder.

NeuroImage. Clinical·2021
Same author

A fixed-target platform for serial femtosecond crystallography in a hydrated environment.

IUCrJ·2020
Same author

Reducing off target viral delivery in ovarian cancer gene therapy using a protease-activated AAV2 vector platform.

Journal of controlled release : official journal of the Controlled Release Society·2019
Same author

On farm factors increasing dark cutting in pasture finished beef cattle.

Meat science·2018
Same author

Indices of repetitive behaviour are correlated with patterns of intrinsic functional connectivity in youth with autism spectrum disorder.

Brain research·2018

Area of Science:

  • Endocrinology
  • Cell Biology
  • Molecular Biology

Background:

  • Steroid hormone action is mediated by intracellular receptors within target tissues.
  • Current understanding favors predominant intranuclear localization of these receptors in intact cells.
  • The cellular dynamics and distribution of steroid hormone receptors are areas of active investigation.

Purpose of the Study:

  • To analyze the microsomal estrogen and androgen binding capabilities in rat uterine and prostate tissues.
  • To characterize the properties and potential functions of these microsomal receptor-like proteins.
  • To differentiate microsomal receptors from cytosolic contaminants and determine their subcellular localization.

Main Methods:

  • Analysis of microsomal estrogen and androgen binding in rat uterine and ventral prostate tissue.

Related Experiment Videos

  • Differential ammonium sulfate precipitation to separate cytosolic and microsomal receptor forms.
  • Sucrose gradient subfractionation of subcellular components with binding and enzyme assays.
  • Solubilization studies of microsomal receptors and assessment of residual acceptor sites.

    • Main Results:

    • Identified high-affinity microsomal binding sites for estrogen and androgen.
    • These sites exhibit properties similar to cytosolic receptors but fail to activate DNA binding.
    • Microsomal estrogen-binding proteins also bind progesterone, distinguishing them from other estrogen receptor species.
    • Subcellular fractionation localized major binding activity to endoplasmic reticulum vesicles.
    • Residual microsomes after extraction acted as saturable acceptor sites for cytosolic estrogen-receptor complexes.

    Conclusions:

    • Microsomal receptor-like proteins may modulate estrogen access to nuclear sites.
    • These proteins could form extranuclear complexes influencing non-genomic cellular processes.
    • They might regulate nuclear estrogen-receptor complex concentrations by accepting extranuclear complexes.