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Electroosmosis in human dentine in vitro.

Kanittha Kijsamanmith¹, Noppakun Vongsavan¹, Bruce Matthews²

¹Department of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok, 10400, Thailand.

Archives of Oral Biology

|September 9, 2020

Summary

This summary is machine-generated.

Electroosmosis drives fluid flow through human dentine during iontophoresis, enhancing drug delivery. This process facilitates the transport of both charged and uncharged molecules into the pulp.

Keywords:

Dentinal fluid Dentine Electroosmosis Humans Iontophoresis Smear layer

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Area of Science:

Biomaterials Science
Dental Research
Fluid Dynamics

Background:

Iontophoresis is a technique used to deliver charged molecules into tissues.
Dentine's complex structure presents a barrier to effective drug penetration.
Understanding fluid dynamics in dentine is crucial for optimizing therapeutic delivery.

Purpose of the Study:

To quantify fluid flow in human dentine induced by electroosmosis during iontophoresis.
To investigate the influence of different solutions on electroosmotic flow.
To assess the potential of electroosmosis to enhance iontophoretic drug delivery.

Main Methods:

Experiments conducted on 24 extracted human premolars with exposed dentine.
Fluid flow measured using a capillary connected to the pulp cavity under controlled pressure.
Electrical current applied between electrodes in the dentine cavity and pulp cavity.

Main Results:

Electrical current significantly induced fluid flow through dentine.
Solution composition and dentine etching did not significantly affect flow rates.
Specific inward and outward flow rates were quantified at different current intensities during iontophoresis of lignocaine with epinephrine.

Conclusions:

Electroosmosis is achievable in human dentine.
Electroosmosis can significantly enhance iontophoresis for transporting charged molecules, especially large ones.
This mechanism may enable the transport of uncharged molecules through dentine into the pulp.