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Related Experiment Video

Updated: Dec 9, 2025

High-throughput Screening for Protein-based Inheritance in S. cerevisiae

High-throughput Screening for Protein-based Inheritance in S. cerevisiae

Published on: August 8, 2017

Mechanisms for Curing Yeast Prions.

Lois E Greene¹, Farrin Saba¹, Rebecca E Silberman^1,2

¹Laboratory of Cell Biology, National Heart Lung Blood Institute National Institutes of Health, Bethesda, MD 20892, USA.

International Journal of Molecular Sciences

|September 10, 2020

Summary

This summary is machine-generated.

Prions are infectious proteins that misfold and propagate. Curing yeast prions requires eliminating prion seeds through various mechanisms like inhibiting severing or promoting degradation.

Keywords:

Hsp104 curing mechanism methods prion yeast

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Area of Science:

Biochemistry
Cell Biology
Molecular Biology

Background:

Prions are infectious proteins characterized by misfolded conformations.
In yeast, prions form amyloid fibers (seeds) that propagate through cell division.
Eliminating all prion seeds is essential for curing yeast prions.

Purpose of the Study:

To review and differentiate the various mechanisms involved in curing yeast prions.
To discuss methods used to distinguish between distinct prion curing pathways.

Main Methods:

Inhibition of prion seed severing.
Promoting gradual dissolution of prion seeds.
Asymmetric segregation of prion seeds during cell division.
Enhancing degradation of prion seeds via protein quality control machinery.

Main Results:

Multiple curing mechanisms can operate simultaneously.
The specific mechanisms employed depend on cellular conditions.
Different protein quality control systems contribute to prion curing.

Conclusions:

Understanding these diverse curing mechanisms is crucial for developing effective prion eradication strategies.
Differentiating between these pathways aids in comprehending prion propagation and clearance in yeast.