Method for monitoring singlet oxygen quantum yield in real time by time resolved spectroscopy measurement

The singlet oxygen quantum yield (Φ_Δ) was monitored in real time through time resolved spectroscopy measurement, using gadolinium labeled hematoporphyrin monomethyl ether (Gd-HMME) as photosensitizer. According to the kinetics equations of singlet oxygen generation and reaction, Φ_Δ was related to phosphorescence lifetime (τ_p). Through measuring τ_p of Gd-HMME in different oxygen conditions, the radiation transition property of first exited triplet state (T₁) was monitored; combined with the triplet state quantum yield (Φ_T) determined by linear fitting the Φ_Δ, which was measured in different oxygen content using a relative measurement, Φ_Δ can be determined in real time. The identification of anoxia during the treatment of photodynamic therapy (PDT) by this method is also presented.