Biocompatible fluorescent nanoparticles for pH-sensoring

Area of Science:

• Biotechnology
• Nanotechnology
• Analytical Chemistry

Background:

• Accurate pH monitoring is crucial in biological and chemical systems.
• Development of novel nanosensors offers improved sensitivity and specificity.
• Fluorescent probes are widely used for sensing applications.

Purpose of the Study:

• To create biocompatible and tuneable nanosensors for ratiometric pH measurements.
• To explore the use of fluorescein and sulforhodamine B marked dextran derivatives in nanosensor fabrication.
• To demonstrate the efficacy of these nanosensors in pH determination.

Main Methods:

• Preparation of dextran derivatives marked with fluorescein and sulforhodamine B.
• Purification of the marked dextran derivatives using dialysis.
• Characterization of the resulting nanosensors for their physical and optical properties.
• Validation of nanosensor performance for ratiometric pH measurements.

Main Results:

• Successful synthesis of biocompatible nanosensors.
• Demonstration of tuneable properties for tailored applications.
• Effective ratiometric pH measurements using the developed nanosensors.
• High sensitivity and specificity in pH sensing.

Conclusions:

• Dialysis of marked dextran derivatives yields effective nanosensors for pH monitoring.
• The developed nanosensors are biocompatible and tuneable.
• This approach offers a promising platform for advanced ratiometric pH sensing in various fields.