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Bio-protocols for directly forming active encapsulations containing living primary cells
Priyesh Patel1, Scott Irvine2, Jean R McEwan3
1Department of Mechanical Engineering, University College London, Torrington Place, London, United KingdomWC1E 7JE. s.jayasinghe@ucl.ac.uk.
Soft Matter
|September 10, 2020
Summary
Bio-electrosprays and cell electrospinning enable direct handling of living cells. This study demonstrates viable cell encapsulation for biomedical applications, showing over 70% cell viability.
Area of Science:
- Biomedical Engineering
- Cell Biology
- Regenerative Medicine
Background:
- Electrosprays and electrospinning are emerging techniques for manipulating living cells.
- These methods, termed bio-electrosprays and cell electrospinning, show promise in regenerative and therapeutic medicine.
Purpose of the Study:
- To develop and assess submerged bio-electrospray and cell electrospinning protocols for creating cell-bearing encapsulations.
- To evaluate the viability of primary rabbit aorta smooth muscle cells after these processes.
Main Methods:
- Utilized submerged electrospray and electrospinning techniques with primary rabbit aorta smooth muscle cells.
- Assessed cell viability using flow cytometry over a three-week period, comparing results against two control groups.
Main Results:
- Successfully generated cell-bearing encapsulations using the developed protocols.
- Achieved a viable cellular population exceeding 70% post-treatment.
- Demonstrated proof-of-concept for diverse biomedical applications.
Conclusions:
- The developed bio-protocols are effective for creating biologically viable, cell-bearing emulsions.
- These electrified encapsulating approaches hold significant potential for applications in cancer therapy, hormone and diabetic treatments, and cosmetics.
- The study highlights the broad implications of these bio-protocols for advancing life sciences research and applications.
