Related Experiment Video
Updated: Dec 9, 2025
Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
Published on: March 3, 2023
Antibacterial Potential of Jatropha sp. Latex against Multidrug-Resistant Bacteria
Muhammad Evy Prastiyanto1,2, Prayoda Deri Tama3, Ninda Ananda3
1Microbiology Laboratory, Department of Medical Laboratory Technology, Universitas Muhammadiyah Semarang, Kedungmundu Raya Street 18, Semarang 50273, Indonesia.
Objective:
This study was aimed to evaluate the antibacterial activity of the latex of three species members of Jatropha (J. curcas, J. gossypilofia Linn., and J. multifida) against methicillin-resistant Staphylococcus aureus (MRSA), extended-spectrum beta-lactamase- (ESBL-) producing Escherichia coli and ESBL-producing Klebsiella pneumonia, carbapenemase-resistant Enterobacteriaceae (CRE)-E. coli, K. pneumoniae-carbapenemase (KPC), and carbapenemase-resistant Pseudomonas aeruginosa (CRPA).
Method:
The antibacterial activities were calculated based on the inhibition zones using the Mueller-Hinton agar diffusion method, minimum inhibitory concentration (MIC) using Mueller-Hinton broth in a microdilution method, and minimum bactericidal concentration (MBC) using blood agar plate.
Results:
The latex of Jatropha showed antibacterial activities against the MRSA and CRPA. All latex of Jatropha appeared to have the antibacterial activities against MRSA and CRPA in the diffusion method (20.4-23.7 mm and 12-15 mm), MIC (0.19-6.25%, and 25%), and MBC (0.39-12.5% and 50%). Phytochemical screening of latex indicated the presence of flavonoids.
Conclusions:
The latex of J. curcas, J. gossypilofia Linn., and J. multifida has the potential to be developed as antibacterial agents, especially against MRSA and CRPA strain, but further in vivo research and discovery of the mode of its action are required to shed the light on the effects.
Related Concept Videos
01:28Antimicrobial Effectiveness
01:30Development of Antibiotic Resistance
00:57Antibiotic Selection
01:31Defense Against Bacterial Pathogens
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
01:23Antimicrobial Proteins
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
01:18Surface Membrane Barriers
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...