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Related Concept Videos

Ischemic Stroke l: Introduction01:15

Ischemic Stroke l: Introduction

Ischemic stroke is an acute cerebrovascular condition in which blood flow to a brain region is suddenly interrupted, leading to tissue infarction. Neurons depend on continuous oxygen and glucose supply, so even brief reductions in perfusion cause energy failure, ionic imbalance, and irreversible injury. Ischemic strokes are classified into thrombotic and embolic types based on their underlying mechanisms.Thrombotic MechanismsThrombotic stroke develops when a clot forms within a cerebral artery.
Ischemic Stroke ll: Pathophysiology01:15

Ischemic Stroke ll: Pathophysiology

An ischemic stroke occurs when a cerebral blood vessel becomes obstructed, most often by a thrombus or embolus, interrupting the delivery of oxygen and glucose to brain tissue. Because neurons rely on continuous aerobic metabolism, energy failure begins within minutes of reduced perfusion. The region receiving the least blood flow becomes the infarct core, an area of irreversible cellular death. Surrounding this core lies the penumbra, a zone of hypoperfused but still viable tissue that is...

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Related Experiment Video

Updated: Jun 26, 2026

Stereological and Flow Cytometry Characterization of Leukocyte Subpopulations in Models of Transient or Permanent Cerebral Ischemia
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Systematic Study of the Immune Components after Ischemic Stroke Using CyTOF Techniques.

Yaning Li1, Yan Wang1, Yang Yao1

  • 1Department of Neurosurgery, School of Medicine, Stanford University, Stanford, CA 94305, USA.

Journal of Immunology Research
|September 10, 2020
PubMed
Summary

Stroke triggers significant immune cell changes in the brain and body. Mass cytometry reveals dynamic immune responses and leukocyte migration into the ischemic brain, offering a comprehensive view of stroke-induced inflammation.

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Area of Science:

  • Neuroimmunology
  • Stroke Pathophysiology
  • Inflammation Research

Background:

  • Stroke elicits a significant inflammatory response, but traditional methods like fluorescence-activated cell sorting (FACS) limit a comprehensive analysis of immune cell populations.
  • A detailed understanding of immune cell dynamics and interactions post-stroke is crucial for developing effective therapeutic strategies.

Purpose of the Study:

  • To systematically characterize immune cell populations and their dynamic changes in the brain and peripheral organs following ischemic stroke.
  • To investigate the expression of cell surface markers associated with inflammation and leukocyte migration.
  • To identify network interactions between immune cells in the central nervous system and peripheral organs after stroke.

Main Methods:

  • Utilized mass cytometry (CyTOF) for high-dimensional analysis of immune cells in the ischemic brain, peripheral blood, spleen, and bone marrow at various time points post-stroke.
  • Analyzed cell surface marker expression to assess inflammation status and identify key molecules like CD62L involved in leukocyte migration.
  • Employed R programming for network analysis to identify cross-organ immune cell interactions.

Main Results:

  • Demonstrated dynamic alterations in immune cell numbers within the ischemic brain and peripheral organs after stroke.
  • Observed distinct patterns of cell surface marker expression, indicating the inflammatory state and the role of molecules like CD62L in immune cell trafficking.
  • Identified a significant leukocyte network connecting the brain and peripheral immune organs as early as day 1 post-ischemia, highlighting rapid migration of peripheral immune cells to the ischemic site.

Conclusions:

  • Mass cytometry provides a comprehensive view of the immune response to ischemic stroke, surpassing the limitations of traditional techniques.
  • Peripheral immune cell migration into the ischemic brain is a rapid and significant event following stroke.
  • This study enhances our understanding of the complex interplay between the central nervous system and peripheral immune system in the context of stroke.