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Updated: Dec 9, 2025

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Improvements to the ARTIC multiplex PCR method for SARS-CoV-2 genome sequencing using nanopore.

John R Tyson¹, Phillip James², David Stoddart²

¹Michael Smith Laboratories and Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, Canada.

Biorxiv : the Preprint Server for Biology

|September 10, 2020

Summary

This study improves a popular SARS-CoV-2 genome sequencing method. Enhancements include better genome coverage, faster library preparation, and reduced costs for widespread genomic epidemiology.

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Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Published on: March 11, 2020

Area of Science:

Virology
Genomics
Epidemiology

Background:

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genome sequencing is crucial for tracking viral evolution and transmission.
Over 90,000 SARS-CoV-2 genome sequences are available in the GISAID database.
An accessible sequencing protocol was previously established in January 2020.

Approach:

An updated primer scheme with 22 additional primers enhances genome coverage.
A streamlined library preparation workflow improves the demultiplexing rate for up to 96 samples.
Reduced hands-on time and reagent costs to approximately £10 per sample.

Key Points:

Improved SARS-CoV-2 genome sequencing protocol.
Enhanced genome coverage and higher demultiplexing rates.
Cost-effective method supporting large-scale genomic epidemiology.

Conclusions:

The improved protocol makes SARS-CoV-2 genome sequencing more efficient and affordable.
This facilitates broader participation in national and international genomic epidemiology efforts.
Supports continuous monitoring of viral evolution and public health surveillance.