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Updated: Dec 9, 2025

A Colorimetric Method for Measuring Iron Content in Plants
Published on: September 7, 2018
Case study on the use of image analysis for the simple and inexpensive colorimetric detection of Fe(iii) in water
Bhooma Bhagat1, Vrajrajsinh Jadeja, Paawan Sharma
1Department of Science, Pandit Deendayal Petroleum University, Gandhinagar-382007, Gujarat, India. kalisadhanm@yahoo.com kalisadhan.mukherjee@sot.pdpu.ac.in.
Abstract:
In the present work, we have reported a simple and cost effective colorimetric method for the detection of Fe(iii) in water. The method is based on the color change through the formation of an Fe(iii)-glycine complex at room temperature. This type of complex formation produces an intense color due to the ligand to metal charge transfer (LMCT). The rate of this type of complex formation depends appreciably on the Fe(iii) concentrations. An important aspect of the present work is that here the image analysis technique has been used successfully for the discrimination of the color obtained by the variation of the Fe(iii) concentration. The fundamental spectro-photochemical studies on the colorimetric detection of Fe(iii) by forming a metal ligand complex and thereafter the discrimination of the complex through image analysis can provide effective insight into the development of cost effective devices for the detection of liquid phase analytes.
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