Related Experiment Video

Updated: Dec 9, 2025

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

8.6K

The transcriptional factors CDX2 and FOXA1 in chordomas

Maroa Dridi1, Jean Boutonnat2, Jean Marc Dumollard1

  • 1Pathology Department, University Hospital of Saint-Etienne, France.

Pathology, Research and Practice
|September 10, 2020
PubMed

Insights

CDX2 is not expressed in chordoma bone tumors, while a limited subset of chordomas show FOXA1 expression. This study investigated these factors in 57 chordomas.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Chordomas are rare bone tumors originating from notochordal remnants.
  • Brachyury activation is implicated in chordoma development.
  • CDX2 and FOXA1 are transcription factors induced by brachyury.

Purpose of the Study:

  • To investigate the expression of CDX2 and FOXA1 in chordomas.
  • To determine if CDX2 or FOXA1 expression correlates with clinical factors in chordoma patients.

Main Methods:

  • Retrospective analysis of 57 chordoma tumor samples.
  • Immunohistochemical staining for CDX2 and FOXA1 expression.
  • Correlation analysis between marker expression and clinical data.

Main Results:

  • CDX2 expression was not detected in any of the studied chordomas.
  • FOXA1 expression was observed in 7 out of 57 (12.3%) chordomas.
  • No significant association was found between FOXA1 expression and clinical parameters.

Conclusions:

  • CDX2 is not a characteristic marker for chordomas.
  • Limited expression of FOXA1 occurs in a subset of chordomas, but lacks clinical correlation.
  • Further research may explore the role of FOXA1 in specific chordoma subsets.
Keywords:
ChondrosacromaChordomaDifferential diagnosisEndodermImmunohistochemistryMesodermNotochord

More Related Videos

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis09:58

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

Published on: June 27, 2020

3.0K
Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis09:20

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Published on: December 18, 2019

7.4K

Related Concept Videos

Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.5K
General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
6.4K
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
81.7K
Combinatorial Gene Control02:33

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
9.3K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
2.0K
Determination01:51

Determination

During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
20.4K