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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Dec 9, 2025

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Profiling cellular heterogeneity in asthma with single cell multiparameter CyTOF.

Emma Stewart¹, Xiaomei Wang¹, Geoffrey L Chupp¹

¹Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut, USA.

Journal of Leukocyte Biology

|September 10, 2020

Summary

Mass cytometry revealed distinct airway immune cell subtypes in asthma patients, particularly neutrophils. This advanced analysis offers new insights into asthma inflammation and potential therapeutic targets.

Keywords:

dendritic cell eosinophil inflammation mass cytometry neutrophil

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Last Updated: Dec 9, 2025

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

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Published on: October 25, 2018

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Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Published on: June 26, 2018

Area of Science:

Immunology
Respiratory Medicine
Cell Biology

Background:

Asthma affects millions globally, characterized by airway inflammation involving various immune cells in sputum.
Current sputum analysis lacks resolution to fully understand immune cell heterogeneity in asthma.
Identifying distinct inflammatory cell phenotypes is crucial for targeted asthma management and therapies.

Purpose of the Study:

To utilize mass cytometry for high-resolution analysis of airway immune cells in asthma.
To define the frequency and functional status of sputum-derived cells in asthmatic patients and healthy controls.
To uncover novel immune cell subtypes and their roles in asthma pathogenesis.

Main Methods:

Mass cytometry (cytometry by time-of-flight) was employed for in-depth single-cell analysis.
Quantification of immune cell frequency and functional status in sputum.
Statistical analysis and unbiased clustering identified distinct cellular phenotypes.

Main Results:

Identified multiple distinct subtypes of airway immune cells, with notable diversity within neutrophils.
Demonstrated differences in cellular function and status among asthmatic patients.
Unbiased clustering revealed significant variations in immune cell populations.

Conclusions:

High-resolution single-cell analysis via mass cytometry provides deeper insights into asthma inflammation.
Distinct immune cell subtypes, especially neutrophils, may contribute to asthma heterogeneity and clinical responses.
This approach can identify new pathways for asthma treatment and research.