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Using genomic resources for linkage analysis in Peromyscus with an application for characterizing Dominant Spot
Zhenhua Shang1, David J Horovitz1, Ronald H McKenzie1
1Department of Biological Sciences, University of South Carolina, Columbia, SC, 29208, USA.
BMC Genomics
|September 11, 2020
Summary
Researchers identified the gene Sox10 linked to the Dominant spot trait in Peromyscus mice. This finding, using genetic analysis of closely related species, advances understanding of coat color variation and the neural crest gene regulatory network.
Area of Science:
- Genetics
- Developmental Biology
- Mammalian Genomics
Background:
- Peromyscus mice are common North American mammals utilized in research.
- Closely related species, P. maniculatus and P. polionotus, produce fertile hybrids, enabling genetic studies.
- Developing genomic resources support linkage analysis for trait-associated genomic loci.
Purpose of the Study:
- To identify DNA polymorphisms between P. maniculatus and P. polionotus.
- To analyze genetic complexity underlying species differences in physiology and behavior.
- To conduct a candidate gene linkage analysis for the Dominant spot trait.
Main Methods:
- Utilized available genomic data to identify DNA polymorphisms.
- Performed candidate gene linkage analysis for the Dominant spot trait.
- Analyzed quantitative variation in spot size based on genetic background.
Main Results:
- Identified DNA polymorphisms between P. maniculatus and P. polionotus.
- Linked the Dominant spot trait to chromosome 20, implicating the Sox10 gene.
- Observed quantitative variation in spot size influenced by genetic background.
Conclusions:
- Peromyscus genomic resources facilitate linkage analysis for trait identification.
- The Dominant spot phenotype is linked to Sox10, a neural crest gene regulatory network member.
- Peromyscus serves as a model for identifying new alleles in the neural crest gene regulatory network.
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