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Updated: Dec 9, 2025

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins
Published on: September 28, 2012
Functions of TopBP1 in preserving genome integrity during mitosis
Jonas Bagge1, Vibe H Oestergaard1, Michael Lisby1
1Department of Biology, University of Copenhagen, DK-2200 Copenhagen N, Denmark.
Abstract:
TopBP1/Rad4/Dpb11 is an essential eukaryotic protein with important roles in DNA replication, DNA repair, DNA damage checkpoint activation, and chromosome segregation. TopBP1 serves as a scaffold to assemble protein complexes in a phosphorylation-dependent manner via its multiple BRCT-repeats. Recently, it has become clear that TopBP1 is repurposed to scaffold different processes dependent on cell cycle regulated changes in phosphorylation of client proteins. Here we review the functions of human TopBP1 in maintaining genome integrity during mitosis.
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