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Author Spotlight: Modeling an Aspect of Preeclampsia in Female Mice Using Hypoxic Human Placenta-Derived Small Extracellular Vesicles
05:31

Author Spotlight: Modeling an Aspect of Preeclampsia in Female Mice Using Hypoxic Human Placenta-Derived Small Extracellular Vesicles

Published on: January 26, 2024

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The pseudogene URAHP promotes proliferation and regulates the pathogenesis of preeclampsia

Weiwei Li1, Yanshu Li2

  • 1Department of Obstetrics, First Hospital of China Medical University Shenyang, China.

American Journal of Translational Research
|September 11, 2020
PubMed

Insights

This study reveals that the pseudogene URAHP, a long noncoding RNA, is upregulated in preeclampsia (PE) placentae and influences cell proliferation, offering a potential new therapeutic target for PE.

Area of Science:

  • Reproductive biology
  • Genomics
  • Molecular medicine

Background:

  • Preeclampsia (PE) is a pregnancy-specific disorder with no effective treatments.
  • Long noncoding RNAs (lncRNAs) are increasingly recognized for their roles in disease pathogenesis.
  • The pseudogene URAHP shows elevated expression in PE placentae.

Purpose of the Study:

  • To investigate the role of the lncRNA URAHP in preeclampsia.
  • To explore the regulatory mechanisms and functional impact of URAHP in placental cells.
  • To identify potential therapeutic targets for preeclampsia.

Main Methods:

  • Differential expression analysis of lncRNAs and mRNAs in normal pregnancy (NP) and PE placentae using microarray.
  • Validation of URAHP expression via quantitative real-time PCR (qRT-PCR).
  • Functional assays (CCK-8, 3D colony formation) to assess URAHP's effect on cell proliferation.
  • Gene coexpression analysis and western blotting to investigate URAHP and KISS1R interactions.

Main Results:

  • High-throughput sequencing identified 675 differentially expressed lncRNAs and 205 differentially expressed genes between PE and NP samples.
  • URAHP expression was significantly increased in PE placentae compared to NP.
  • URAHP downregulation affected cell proliferation, while URAHP overexpression promoted it; URAHP and KISS1R were found to be coexpressed.

Conclusions:

  • The pseudogene URAHP is implicated in preeclampsia for the first time.
  • URAHP represents a novel molecular target for preeclampsia treatment strategies.
Abstract

Keywords:
KISS1RURAHPpreeclampsiapseudogene

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