Circ_0007142/miR-186/FOXK1 axis promoted lung adenocarcinoma progression
Dongjie Ma1, Hongsheng Liu1, Yingzhi Qin1
1Department of Thoracic Surgery, Peking Union Medical College Hospital Beijing 100730, China.
American Journal of Translational Research
|September 11, 2020
Summary
Circular RNAs (circRNAs) are key in lung adenocarcinoma. Circ_0007142 promotes tumor growth and spread by affecting the miR-186/FOXK1 axis and Wnt/β-catenin pathway.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Circular RNAs (circRNAs) are non-coding RNAs implicated in gene expression regulation.
- Aberrant circRNA expression is linked to cancer development and progression.
- Lung adenocarcinoma (LUAD) pathogenesis requires further elucidation of molecular mechanisms.
Purpose of the Study:
- To identify differentially expressed circRNAs in lung adenocarcinoma.
- To investigate the role of circ_0007142 in LUAD progression.
- To elucidate the molecular mechanisms underlying circ_0007142's function in LUAD.
Main Methods:
- CircRNA microarray analysis of LUAD and adjacent normal tissues.
- Quantitative real-time PCR to validate circRNA expression.
- Cell proliferation, migration, invasion, and apoptosis assays following circ_0007142 knockdown.
Main Results:
- 32 differentially expressed circRNAs were identified between LUAD and normal tissues.
- Circ_0007142 was significantly upregulated in LUAD tissues and cells.
- Knockdown of circ_0007142 suppressed LUAD cell proliferation, migration, and invasion, while inducing apoptosis.
- Circ_0007142 knockdown affected LUAD progression via the miR-186/FOXK1 axis and Wnt/β-catenin pathway inactivation.
Conclusions:
- Circ_0007142 is a key oncogenic circRNA in lung adenocarcinoma.
- The circ_0007142/miR-186/FOXK1 axis represents a novel regulatory mechanism in LUAD.
- Targeting circ_0007142 may offer a potential therapeutic strategy for lung adenocarcinoma.
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