Jove
Visualize
Contact Us

Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

851
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
851
Electrophoresis: Overview01:20

Electrophoresis: Overview

3.2K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
3.2K
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

682
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
682
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

1.5K
Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
1.5K
Affinity Chromatography01:03

Affinity Chromatography

2.5K
Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
2.5K
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

1.4K
In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
1.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>Trans</i>-cyclosulfamidate mannose-configured cyclitol allows isoform-dependent inhibition of GH47 α-d-mannosidases through a bump-hole strategy.

Chemical science·2023
Same author

Higher Plasma Levels of Endocannabinoids and Analogues Correlate With a Worse Cardiometabolic Profile in Young Adults.

The Journal of clinical endocrinology and metabolism·2023
Same author

In vitro metabolism of cathinone positional isomers: does sex matter?

Analytical and bioanalytical chemistry·2023
Same author

A bout of endurance and resistance exercise transiently decreases plasma levels of bile acids in young, sedentary adults.

Scandinavian journal of medicine & science in sports·2023
Same author

The relative abundance of fecal bacterial species belonging to the Firmicutes and Bacteroidetes phyla is related to plasma levels of bile acids in young adults.

Metabolomics : Official journal of the Metabolomic Society·2023
Same author

Urinary Metabolic Profiling in Volunteers Undergoing Malaria Challenge in Gabon.

Metabolites·2022
Same journal

Kinship Inferences for Second-Degree Relatives With a Combination of STRs and Microhaplotypes.

Electrophoresis·2026
Same journal

Optimisation of Electrokinetic Extraction System: Colourimetric Determination of Copper (II) in Sand Using Polymer Inclusion Membrane.

Electrophoresis·2026
Same journal

Novel Phloroglucinol Derivatives as Neuraminidase Inhibitors Identified From Humulus lupulus L. Extract by At-Line Nanofractionation Platform.

Electrophoresis·2026
Same journal

Protein-Based High-Performance Liquid Chromatography and Cyclodextrin-Capillary Electrokinetic Chromatography for the Chiral Separation of Azoles.

Electrophoresis·2026
Same journal

Dynamics of Heparin Translocations Through Solid-State Nanopores.

Electrophoresis·2026
Same journal

Production of Protein Hydrolysates and Bioactive Peptides From Lablab purpureus and Macrotyloma uniflorum via Optimized Extraction and Proteolysis Protocols.

Electrophoresis·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Dec 9, 2025

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
14:12

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

2.3K

Past, present, and future developments in enantioselective analysis using capillary electromigration techniques.

Nicky de Koster1, Charles P Clark1, Isabelle Kohler2

  • 1Leiden Academic Centre for Drug Research, Division of Systems Biomedicine and Pharmacology, Leiden University, Leiden, The Netherlands.

Electrophoresis
|September 11, 2020
PubMed
Summary
This summary is machine-generated.

Chiral capillary electrophoresis (CE) offers a cost-effective and sensitive method for separating chiral compounds. Advances in CE, including CE-MS, enhance chiral analysis in various applications.

Keywords:
CE-MSChiral CECChiral analysisChiral electrokinetic chromatographyEnantioselective separation

More Related Videos

Sheathless Capillary Electrophoresis&#8211;Mass Spectrometry for Metabolic Profiling of Biological Samples
07:46

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Published on: October 1, 2016

12.0K
Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
10:05

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

9.9K

Related Experiment Videos

Last Updated: Dec 9, 2025

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
14:12

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

2.3K
Sheathless Capillary Electrophoresis&#8211;Mass Spectrometry for Metabolic Profiling of Biological Samples
07:46

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Published on: October 1, 2016

12.0K
Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
10:05

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

9.9K

Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Chiral enantioseparation is crucial for academic and industrial applications.
  • Separating chiral compounds requires high-resolution, sensitive analytical techniques.
  • Capillary Electrophoresis (CE) has demonstrated significant success in chiral analysis.

Purpose of the Study:

  • To review the advancements and potential of Capillary Electrophoresis (CE) for chiral separations.
  • To highlight the benefits and challenges of various CE techniques, including chiral EKC and chiral CEC.
  • To explore the integration of Mass Spectrometry (MS) with CE for enhanced chiral analysis.

Main Methods:

  • Enantioseparation using chiral Electrokinetic Chromatography (EKC) with diverse pseudostationary phases.
  • Chiral Capillary Electrochromatography (CEC) combining chromatographic principles with CE fluid dynamics.
  • Capillary Electrophoresis-Mass Spectrometry (CE-MS) for sensitive and selective chiral detection.

Main Results:

  • Chiral CE provides orthogonal enantioselectivity and is cost-effective compared to chromatography.
  • Chiral EKC offers flexibility through various pseudostationary phases.
  • Chiral CEC reduces band broadening compared to pressure-driven systems.
  • CE-MS enhances sensitivity and selectivity for complex chiral sample analysis, with techniques to mitigate ion source contamination.

Conclusions:

  • CE, particularly CE-MS, is a powerful and adaptable technique for chiral analysis.
  • Ongoing developments in pseudostationary phases, chiral ionic liquids, polymers, and frameworks will further establish CE(-MS) for routine chiral separations.
  • Further method validation is needed for monolithic and open-tubular CEC-MS applications.