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Related Concept Videos

Labeling DNA Probes03:31

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging
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A Dendron-Based Fluorescence Turn-On Probe for Tumor Detection.

Changren Liu1, Ling'e Zhang1, Sensen Zhou1

  • 1Department of Polymer Science & Engineering, College of Chemistry & Chemical Engineering, Nanjing University, Nanjing, 210023, P.R. China.

Chemistry (Weinheim an Der Bergstrasse, Germany)
|September 11, 2020
PubMed
Summary

This study introduces novel dendron-based probes that enhance tumor imaging sensitivity. These probes utilize a fluorescence turn-on mechanism activated in tumor environments, improving cancer detection.

Keywords:
Förster resonance energy transferdendronsfluorescence turn-on probesreductive responsetumor imaging

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Organic Chemistry

Background:

  • Optical probes are crucial for sensitive tumor imaging.
  • Current probes often lack sufficient sensitivity and contrast.
  • Amplifying emission signals in tumors is key to improved detection.

Purpose of the Study:

  • To develop and characterize novel dendron-based fluorescence turn-on probes.
  • To investigate the Förster resonance energy transfer (FRET) mechanism for probe activation.
  • To evaluate the probe's efficacy in tumor imaging in vitro and in vivo.

Main Methods:

  • Synthesis of fourth-generation dendrons with oligo(ethylene glycol) scaffolds via solid-phase synthesis.
  • Conjugation of a Cy5.5 fluorophore and eight Black Hole Quencher 3 (BHQ-3) molecules to the dendron structure.
  • Utilizing a reductively cleavable disulfide linkage for controlled probe activation.
  • In vitro and in vivo experiments to assess probe performance in tumor environments.

Main Results:

  • Dendron-based probes exhibit precise, defect-free chemical structures.
  • Fluorescence is rapidly activated in the reductive environments of tumor cells and tissues.
  • The probes show amplified tumor signals and reduced normal tissue signals.
  • Demonstrated advantages over existing nanoscale probes, including well-defined structures and controllable conjugation.

Conclusions:

  • Dendron-based fluorescence turn-on probes offer a promising new strategy for enhanced tumor detection.
  • The FRET-mediated mechanism and specific design enable sensitive and selective tumor imaging.
  • The probes possess desirable chemical stability and reproducible pharmacokinetic profiles for clinical translation.