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Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...

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Related Experiment Video

Updated: Dec 9, 2025

Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

Published on: February 16, 2022

How ADA methodology informs SARS-CoV-2 assay development

Caroline Becker¹

¹Translational Medicine Department, QPS LLC, Delaware Technology Park, 3 Innovation Way, Suite 240, Newark, DE 19711, USA.

|September 11, 2020

Summary

No abstract available in PubMed .

Keywords:

COVID-19 SARS-CoV-2 antidrug antibody assay coronavirus rRT-PCR serology

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