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Updated: Dec 9, 2025

Synthesis and Purification of Iodoaziridines Involving Quantitative Selection of the Optimal Stationary Phase for Chromatography
Published on: May 16, 2014
A novel profuse color card for convenient visual determination of iodide in human urine based on catalytic oxidation
Dandan Ren1, Jie Mei1, Jian Bao1
1School of Pharmacy, Nanjing Medical University, Nanjing, Jiangsu 211166, PR China.
Abstract:
In this work, we reported a novel and convenient profuse color card for naked eye determination of iodide (I-) in urine using chromogenic substrate 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). I- catalyzed the oxidation of ABTS by peroxyacetic acid causing ABTS to yield cyan product ABTS+ with a new absorption peak at 730 nm. The addition of rose-red dye rhodamine B (RhB) changes the overall color of the solution from pink to purple and finally to blue, which makes the solution multicolor and easy to distinguish. A good linear relationship for I- was obtained ranging from 10.0 to 500.0 μg/L with the detection limit of 9.2 μg/L. Importantly, the sensor can semi-quantitatively estimate the concentration of I- in human urine with naked eye through the standard color card and assess the deficiency or excess of iodine in human body. The proposed profuse color card opens up a new colorimetric method for the rapid, simple and reliable determination of I- in clinic, and has promising applications in developing assay kit for the clinical diagnosis of I- in urine.
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