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Updated: Dec 9, 2025

Sampling and Pretreatment of Tooth Enamel Carbonate for Stable Carbon and Oxygen Isotope Analysis
Published on: August 15, 2018
Ancient Beringian paleodiets revealed through multiproxy stable isotope analyses.
Carrin M Halffman1, Ben A Potter2, Holly J McKinney3
1Department of Anthropology, University of Alaska Fairbanks, Fairbanks, AK, USA. cmhalffman@alaska.edu rhovanion25@gmail.com.
Ancient Beringian infants consumed complex diets, including significant amounts of salmon, challenging previous notions of early Native American foraging. This reveals seasonal dietary structures in prehistoric populations.
Area of Science:
- Paleoecology
- Paleoanthropology
- Isotope Geochemistry
Background:
- Early Native American diets are debated, with theories of megafaunal specialization versus generalist foraging.
- Faunal records alone are insufficient to resolve dietary debates.
- Stable isotope analysis offers direct insight into consumed foods.
Purpose of the Study:
- To investigate the diets of Ancient Beringian infants using multi-tissue stable isotope analysis.
- To reconstruct maternal dietary patterns during fetal development.
- To test hypotheses about early Holocene foraging strategies in Alaska.
Main Methods:
- Stable isotope analysis (carbon and nitrogen) of infant bone collagen and tooth enamel.
- Multi-tissue analysis to differentiate between infant and maternal diets.
- Modeling of fetal bone turnover and seasonal resource availability.
Main Results:
- Maternal diets were primarily terrestrial but included significant salmon consumption.
- Carbon isotope analysis of essential amino acids and bone bioapatite confirmed the importance of salmon.
- Tooth enamel isotopes indicated increased salmon consumption from spring to summer.
Conclusions:
- Ancient Beringian diets were complex and seasonally structured, not fitting simple megafaunal specialist or generalist forager models.
- Isotope analysis provides crucial data for understanding prehistoric subsistence strategies.
- This study highlights the importance of marine resources in early Holocene Alaska.
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