A promoter assay system using gene targeting in agaricomycetes Pleurotus ostreatus and Coprinopsis cinerea
Dong Xuan Nguyen1, Takehito Nakazawa2, Genki Myo2
1Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan; Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City, Viet Nam.
Journal of Microbiological Methods
|September 12, 2020
Abstract:
A novel promoter assay was developed for Agaricomycetes, using a gene-targeting approach, with or without the CRISPR/Cas9 technique. It enables precise evaluation of promoter activity at the original site of the chromosome without random and multiple integrations in conventional transformation experiments.


