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Updated: Dec 9, 2025
Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
Published on: January 22, 2014
Serum exosomal microRNA transcriptome profiling in subacute spinal cord injured rats
Shu-Qin Ding1, Yu-Qing Chen2, Jing Chen2
1Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical College, Anhui 233004, PR China; Anhui Key Laboratory of Tissue Transplantation, The First Affiliated Hospital of Bengbu Medical College, Anhui 233004, PR China.
Abstract:
MicroRNAs (miRNAs) are involved in a series of pathology of spinal cord injury (SCI). Although, locally expressed miRNAs have advantages in studying the pathological mechanism, they cannot be used as biomarkers. The "free circulation" miRNAs can be used as biomarkers, but they have low concentration and poor stability in body fluids. Exosomal miRNAs in body fluids have many advantages comparing with free miRNAs. Therefore, we hypothesized that the specific miRNAs in the central nervous system might be transported to the peripheral circulation and concentrated in exosomes after injury. Using next-generation sequencing, miRNA profiles in serum exosomes of sham and subactue SCI rats were analyzed. The results showed that SCI can lead to changes of serum exosomal miRNAs. These changed miRNAs and their associated signaling pathways may explain the pathological mechanism of suacute SCI. More importantly, we found some valuable serum exosomal miRNAs for diagnosis and prognosis of SCI.
Insights
Spinal cord injury (SCI) alters specific microRNAs (miRNAs) within circulating exosomes. These exosomal miRNAs show promise as diagnostic and prognostic biomarkers for SCI, offering a new avenue for clinical assessment.
Area of Science:
- Neuroscience
- Biochemistry
- Molecular Biology
Background:
- MicroRNAs (miRNAs) play a role in spinal cord injury (SCI) pathology.
- Locally expressed miRNAs are difficult to use as biomarkers, while circulating free miRNAs are unstable.
- Exosomal miRNAs offer advantages as stable biomarkers in body fluids.
Purpose of the Study:
- To investigate if central nervous system-specific miRNAs are transported to peripheral circulation and concentrated in exosomes after SCI.
- To identify potential serum exosomal miRNA biomarkers for SCI diagnosis and prognosis.
Main Methods:
- Serum exosomal miRNA profiles were analyzed using next-generation sequencing in sham and SCI rats.
- Comparison of miRNA expression between injured and control groups.
Main Results:
- Spinal cord injury significantly altered serum exosomal miRNA profiles.
- Identified specific miRNAs and associated signaling pathways potentially involved in SCI pathology.
- Discovered valuable serum exosomal miRNAs for SCI diagnosis and prognosis.
Conclusions:
- Serum exosomal miRNAs are altered following SCI and reflect pathological changes.
- Specific exosomal miRNAs hold potential as non-invasive biomarkers for SCI diagnosis and prognosis.
