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Updated: Dec 9, 2025
Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells
Published on: August 23, 2014
Generation of an ESRG Pr-tdTomato reporter human embryonic stem cell line, CSUe011-A, using CRISPR/Cas9 editing
Hui Liu1, Shasha Li2, Caiping Ren2
1Cancer Research Institute, Department of Neurosurgery, School of Basic Medical Science, Xiangya Hospital, Central South University, Changsha, China; Laboratory of Stem Cell & Retinal Regeneration, Institute of Stem Cell Research, Division of Ophthalmic Genetics, The Eye Hospital, Wenzhou Medical University, Wenzhou, China; The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health and the Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Central South University, Changsha, China.
Abstract:
ESRG was first identified in our previous study. It is highly expressed in human embryonic stem cells (hESCs), whereas it is significantly down-regulated in differentiated cells and is undetectable in adult tissues. To develop an hESC line for monitoring the expression of ESRG for further study of its function, we used gene editing techniques to insert fusion sequences of ESRG promoter and tdTomato fluorescent protein gene into the AAVS1 human safe harbor locus. The gene-edited line had a normal karyotype, expressed pluripotency markers and differentiation potential.
