Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Surgical Technique and Outcomes of Selective Tibial Nerve Transfer Surgery in Foot Drop Patients.

Plastic and reconstructive surgery. Global open·2026
Same author

The management of sternal mediastinitis in heart and lung transplant patients: An 8-year experience and application of reconstructive algorithm.

Journal of plastic, reconstructive & aesthetic surgery : JPRAS·2026
Same author

The Role of Flow Couplers in Free Flap Monitoring: Evidence From a Systematic Review and Meta-analysis.

Plastic and reconstructive surgery. Global open·2026
Same author

Perforator Versus Non-perforator Flap for Perineal Reconstruction: Long-Term Comparison of Complications and Quality of Life.

Annals of surgical oncology·2026
Same author

ASO Visual Abstract: Perforator Versus Non-perforator Flap for Perineal Reconstruction-Long-Term Comparison of Complications and Quality of Life.

Annals of surgical oncology·2026
Same author

Editorial. Restoration of the balance: a shared art in hand functionality.

The Journal of hand surgery, European volume·2026

Related Experiment Video

Updated: Dec 9, 2025

Procurement and Perfusion-Decellularization of Porcine Vascularized Flaps in a Customized Perfusion Bioreactor
10:56

Procurement and Perfusion-Decellularization of Porcine Vascularized Flaps in a Customized Perfusion Bioreactor

Published on: August 1, 2022

3.1K

Development of vascularized nerve scaffold using perfusion-decellularization and recellularization.

Tsering Wüthrich1, Ioana Lese2, David Haberthür3

  • 1Department for BioMedical Research, University of Bern, Switzerland.

Materials Science & Engineering. C, Materials for Biological Applications
|September 13, 2020
PubMed
Summary

Perfusion-decellularization creates vascularized nerve grafts (VNG) preserving extracellular matrix and vasculature. This bioengineering approach may improve peripheral nerve regeneration for large defects.

Keywords:
Extracellular matrixPerfusion-decellularizationPeripheral nerve injuriesTissue engineeringVascularized nerve

More Related Videos

Decellularization and Recellularization Methodology for Human Saphenous Veins
11:35

Decellularization and Recellularization Methodology for Human Saphenous Veins

Published on: July 27, 2018

14.6K
Two Methods for Decellularization of Plant Tissues for Tissue Engineering Applications
05:20

Two Methods for Decellularization of Plant Tissues for Tissue Engineering Applications

Published on: May 31, 2018

15.1K

Related Experiment Videos

Last Updated: Dec 9, 2025

Procurement and Perfusion-Decellularization of Porcine Vascularized Flaps in a Customized Perfusion Bioreactor
10:56

Procurement and Perfusion-Decellularization of Porcine Vascularized Flaps in a Customized Perfusion Bioreactor

Published on: August 1, 2022

3.1K
Decellularization and Recellularization Methodology for Human Saphenous Veins
11:35

Decellularization and Recellularization Methodology for Human Saphenous Veins

Published on: July 27, 2018

14.6K
Two Methods for Decellularization of Plant Tissues for Tissue Engineering Applications
05:20

Two Methods for Decellularization of Plant Tissues for Tissue Engineering Applications

Published on: May 31, 2018

15.1K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Vascularized nerve grafts (VNG) show promise for peripheral nerve regeneration, especially in large defects.
  • Current VNG limitations include surgical complexity, donor site morbidity, and limited availability.
  • Perfusion-decellularization offers a potential solution for bioengineering VNG.

Purpose of the Study:

  • To explore perfusion-decellularization for creating bioengineered vascularized nerve grafts.
  • To assess the preservation of vascular structures and extracellular matrix (ECM) post-decellularization.
  • To evaluate the potential of these scaffolds for peripheral nerve reconstruction.

Main Methods:

  • Porcine sciatic nerves with vascular pedicles were decellularized using perfusion.
  • Preservation of ECM, vascular patency, and cytokines was analyzed.
  • Scaffolds were reendothelialized using porcine aortic endothelial cells in a perfusion-bioreactor.

Main Results:

  • Decellularization effectively removed cells while preserving ECM and nerve fascicle structure.
  • 3D micro-CT imaging confirmed excellent preservation of vasculature, including capillaries.
  • Measurable growth factors remained, and endothelial cells successfully engrafted into large vessels.

Conclusions:

  • Perfusion-decellularization successfully generates vascularized nerve scaffolds with preserved ECM and vasculature.
  • Engineered vascularized nerve scaffolds show potential for enhanced nerve regeneration compared to non-vascularized conduits.
  • This method offers a promising approach for reconstructing large peripheral nerve defects.