SNHG10 Promotes Cell Proliferation and Migration in Gastric Cancer by Targeting miR-495-3p/CTNNB1 Axis

Area of Science:

• Oncology
• Molecular Biology
• Gene Regulation

Background:

• Long non-coding RNAs (lncRNAs) are key regulators in human cancers, including gastric cancer (GC).
• Small nucleolar RNA host gene 10 (SNHG10) is implicated as an oncogene in various cancers, but its role in GC remains unelucidated.
• Investigating lncRNAs like SNHG10 is crucial for understanding GC pathogenesis.

Purpose of the Study:

• To investigate the functional role of SNHG10 in gastric cancer (GC) cells.
• To elucidate the underlying molecular mechanism through which SNHG10 influences GC progression.
• To determine the relationship between SNHG10, miR-495-3p, and CTNNB1 in GC.

Main Methods:

• Quantitative real-time PCR (RT-qPCR) to measure SNHG10, miR-495-3p, and CTNNB1 expression.
• Loss-of-function assays (CCK-8, colony formation, flow cytometry, Transwell) to assess SNHG10's impact on GC cell proliferation, apoptosis, migration, and invasion.
• Mechanism experiments to identify downstream targets and pathways regulated by SNHG10.

Main Results:

• SNHG10 expression was significantly upregulated in GC cells.
• Knockdown of SNHG10 suppressed GC cell proliferation, migration, and invasion.
• SNHG10 downregulation led to decreased expression of WNT signaling pathway core factors, including CTNNB1, by sequestering miR-495-3p.

Conclusions:

• SNHG10 acts as an oncogene in gastric cancer (GC).
• SNHG10 promotes GC progression by targeting the miR-495-3p/CTNNB1 axis.
• SNHG10 facilitates GC development through the activation of the WNT signaling pathway.