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Three Dimensional Quercetin-Functionalized Patterned Scaffold: Development, Characterization, and In Vitro Assessment
Priya Vashisth1, Neelakshi Kar2, Deepak Gupta2
1Wadhwani Research Centre for Bioengineering, Indian Institute of Technology Bombay, Mumbai, Maharashtra 400076, India.
ACS Omega
|September 14, 2020
Summary
This study developed a novel hybrid scaffold using aligned nanofibers and a hydrogel to guide neural cell growth. The functionalized scaffold shows potential for nerve regeneration and repairing injured neural areas.
Area of Science:
- Biomaterials Science
- Neuroscience
- Tissue Engineering
Background:
- Neural regeneration is challenging due to the nervous system's complexity and limited neural cell repair capacity.
- Patterned and functionalized scaffolds offer a promising strategy to control directional neural cell growth.
Purpose of the Study:
- To fabricate and evaluate a novel patterned hybrid scaffold for enhanced nerve regeneration.
- To investigate the influence of scaffold topology and functionalization on neural cell behavior in vitro.
Main Methods:
- Fabrication of aligned nanofibers (ANFs) via electrospinning.
- Development of a hybrid scaffold (HANF) with ANFs in a gellan-gelatin hydrogel matrix.
- In vitro assessment of neural cell adhesion, growth, and morphology on the functionalized scaffold.
Main Results:
- The HANF scaffold provided mechanical strength and contact guidance for neural cell adhesion and growth.
- Functionalized scaffolds promoted directed neurite outgrowth and favorable cell morphology.
- The fabricated scaffold demonstrated no cytotoxicity toward neural cells.
Conclusions:
- The developed hybrid scaffold shows potential for guiding nerve tissue growth.
- This scaffold can serve as a promising nerve regeneration material for treating injured neural areas.
