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Updated: Dec 9, 2025

In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth
Published on: June 28, 2019
A progesterone receptor membrane component 1 antagonist induces large vesicles independent of progesterone receptor
Lihua Wang-Eckhardt1, Matthias Eckhardt1
1Institute of Biochemistry and Molecular Biology, Medical Faculty, University of Bonn, Nussallee 11, D-53115 Bonn, Germany.
Abstract:
Treatment of different cell lines with progesterone receptor membrane component 1 (PGRMC1) antagonist AG-205 rapidly induces the formation of large vesicular structures that likely represent endosomes. Crispr/Cas9 was used to target the PGRMC1 and progesterone receptor membrane component 2 (PGRMC2) genes in CHO-K1 and HeLa. Unexpectedly, deficiency in one of these or both genes did not inhibit the formation of enlarged vesicles by AG-205, demonstrating additional molecular target(s) of this compound besides PGRMC1. Thus, AG-205 cannot be regarded as a PGRMC1-specific antagonist. However, provided that its currently unknown target(s) will be identified, AG-205 may serve as a new reagent to study endosomal trafficking.
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