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Updated: Dec 9, 2025
In vitro Assessment of Myocardial Protection following Hypothermia-Preconditioning in a Human Cardiac Myocytes Model
Published on: October 27, 2020
Protective effect of inhibiting TRPM7 expression on hypoxia post-treatment H9C2 cardiomyocytes
Juesheng Yang1, Shan Hu2, Li Huang3
1Department of Cardiovascular Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang Jiangxi, China.
Background:
Transient receptor potential channel 7 (TRPM7) plays an important role in maintaining intracellular ion concentration and osmotic pressure.
Objective:
The purpose of this study was to investigate the role and mechanism of inhibiting the expression of TRPM7 in the treatment of distal myocardial ischemia.
Methods:
H9C2 cells were treated with hypoxia post-treatment and reperfusion, respectively, detect the expression of HIF-1α and TRPM7, the concentration of Ca2+ and the degree of apoptosis in the H9C2 cells. The relevant miRNAs targeting TRPM7 were searched, the TRPM7 interference vectors were constructed, and the interference of different interference vectors on TRPM7 in H9C2 cells was detected.
Results:
The results showed that hypoxia post-treatment treatment would lead to increased expression of miR-22-3p which directly targeting TRPM7, decreased expression of TRPM7, increased expression of HIF-α and increased intracellular Ca2+ concentration. While reperfusion can increase the expression of HIF-1α and TRPM7 in H9C2 cells and increase the degree of apoptosis.
Conclusion:
Knockdown of TRPM7 can significantly reduce reperfusion injury in H9C2 cells, reduce the degree of apoptosis, and the TRPM7 interference vector can inhibit the expression of TRPM7 and have a certain protective effect on the reperfusion injury of H9C2 cells.
Insights
Inhibiting Transient Receptor Potential Channel 7 (TRPM7) reduces myocardial reperfusion injury. Knocking down TRPM7 expression protects H9C2 cells from apoptosis and damage, offering a potential therapeutic strategy.
Area of Science:
- Cardiovascular Research
- Molecular Biology
- Cellular Physiology
Background:
- Transient receptor potential channel 7 (TRPM7) is crucial for maintaining intracellular ion balance and osmotic pressure.
- Dysregulation of TRPM7 is implicated in cellular stress responses.
Purpose of the Study:
- To investigate the role of TRPM7 expression inhibition in treating distal myocardial ischemia.
- To elucidate the underlying mechanisms of TRPM7's involvement in myocardial reperfusion injury.
Main Methods:
- H9C2 cells were subjected to hypoxia and reperfusion.
- Expression of HIF-1α, TRPM7, intracellular Ca2+, and apoptosis levels were measured.
- miRNAs targeting TRPM7 were identified, and interference vectors were constructed and tested.
Main Results:
- Hypoxia increased miR-22-3p, decreased TRPM7, and elevated HIF-1α and intracellular Ca2+.
- Reperfusion upregulated HIF-1α and TRPM7, exacerbating apoptosis.
- TRPM7 knockdown via interference vectors reduced TRPM7 expression in H9C2 cells.
Conclusions:
- TRPM7 knockdown significantly mitigates H9C2 cell reperfusion injury and apoptosis.
- TRPM7 interference vectors demonstrate a protective effect against myocardial reperfusion injury by inhibiting TRPM7 expression.
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