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Related Experiment Videos

Root surface debridement--an in vitro assessment.

W A Cheetham1, M Wilson, J B Kieser

  • 1Department of Periodontology, Eastman Dental Hospital, London, England.

Journal of Clinical Periodontology
|May 1, 1988
PubMed
Summary
This summary is machine-generated.

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Extensive root planing may not be necessary for periodontal healing. Studies show minimal cytotoxic lipopolysaccharide (LPS) remains on root surfaces after conservative instrumentation, questioning traditional methods.

Area of Science:

  • Periodontology
  • Biomaterials Science

Background:

  • Root planing aims to create biologically compatible root surfaces for periodontal healing.
  • Recent research questions the necessity of removing all 'diseased cementum' due to minimal cytotoxic material incorporation.

Purpose of the Study:

  • To quantify residual lipopolysaccharide (LPS) on root surfaces after in vitro instrumentation.
  • To evaluate the effectiveness of a conservative root planing protocol in removing LPS.

Main Methods:

  • Limulus amoebocyte lysate (LAL) assay and Polymyxin B affinity chromatography were used.
  • A standardized protocol of 15 instrument strokes per root surface was applied to 18 single-rooted teeth.

Main Results:

  • The conservative instrumentation protocol harvested varying amounts of LPS.

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  • Less than 0.24 ng of LPS per tooth remained in 72% of the tested samples.
  • This indicates significant reduction of LPS with a conservative approach.

    • Conclusions:

    • Extensive root planing may not be required for effective LPS removal.
    • Conservative root surface instrumentation appears sufficient to achieve 'biologically compatible' root surfaces.
    • Findings support a re-evaluation of traditional, aggressive root planing techniques.