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Molecular epidemiology of mumps viruses in the Netherlands, 2017-2019

Rogier Bodewes1, Linda Reijnen1, Jeroen Kerkhof1

  • 1Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands.

Plos One
|September 14, 2020
PubMed

Insights

Molecular epidemiology reveals two mumps virus lineages circulating in the Netherlands between 2017-2019. Analysis of the SH gene and non-coding regions (SH+NCRs) is effective for mumps virus surveillance.

Area of Science:

  • Virology
  • Molecular Epidemiology
  • Public Health

Background:

  • Mumps cases persist globally, even in highly vaccinated populations.
  • The Netherlands experienced mumps outbreaks in 2009-2012, followed by sporadic cases.
  • Molecular epidemiology is crucial for understanding mumps virus transmission dynamics.

Purpose of the Study:

  • To investigate the molecular epidemiology of mumps viruses in the Netherlands from 2017-2019.
  • To compare phylogenetic analyses using near-complete genomes versus SH gene and non-coding regions (SH+NCRs).
  • To assess the utility of SH+NCRs sequencing for molecular surveillance of mumps.

Main Methods:

  • Analysis of Sanger sequence data from SH+NCRs of 82 mumps genotype G viruses.
  • Obtaining near-complete mumps virus genomes from 10 isolates using next-generation sequencing.
  • Phylogenetic analysis comparing trees derived from SH+NCRs and near-complete genomes.

Main Results:

  • Two distinct genetic lineages of mumps genotype G viruses were identified in the Netherlands.
  • Phylogenetic analyses using SH+NCRs and near-complete genomes showed similar topologies.
  • Near-complete genome analysis resulted in slightly longer phylogenetic branches compared to SH+NCRs analysis.
  • SH+NCRs sequence analysis proved effective for molecular surveillance.

Conclusions:

  • The study confirms the utility of SH+NCRs sequencing for molecular surveillance of mumps.
  • Findings suggest potential intermittent circulation of mumps genotype G viruses in the Netherlands during 2017-2019.
  • Molecular data provides valuable insights into mumps virus epidemiology in the region.

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