Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nephrotic Syndrome II : Assessment and Medical Management01:26

Nephrotic Syndrome II : Assessment and Medical Management

114
IntroductionNephrotic syndrome is a kidney disorder marked by excessive protein loss in the urine, leading to various systemic complications. This condition often results from damage to the glomeruli—the kidney's filtering units—causing proteinuria, low blood protein levels, and fluid retention. Understanding the assessment, diagnosis, and management of nephrotic syndrome is essential for effective treatment and prevention of further kidney damage.AssessmentPatient History: Document...
114
Nephrotic Syndrome I : Introduction01:24

Nephrotic Syndrome I : Introduction

324
Nephrotic Syndrome is a chronic kidney disorder defined by clinical findings such as severe proteinuria, hypoalbuminemia, hyperlipidemia, and edema. These symptoms result from damage to the glomeruli, the kidney’s filtering units, increasing their permeability to proteins.Definition and Meaning:Proteinuria, defined as the loss of more than 3.5 grams of protein per day in adults, is a crucial feature of nephrotic syndrome. This condition is often accompanied by edema, the accumulation of...
324
Nephrotic Syndrome III : Nursing Management01:24

Nephrotic Syndrome III : Nursing Management

183
Nursing management for nephrotic syndrome adapts as the disease progresses, with strategies evolving to address advancing symptoms and complications.Early-Stage Management In the early stages, nursing interventions for nephrotic syndrome resemble those used in managing acute glomerulonephritis, focusing on symptom monitoring, fluid balance, and managing mild to moderate edema.Vital Signs: Regularly monitor blood pressure, pulse, respiratory rate, and temperature to promptly identify...
183
Proteomics01:33

Proteomics

9.0K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
9.0K
Serum Studies: Renal Function Tests01:24

Serum Studies: Renal Function Tests

248
Renal function tests are crucial for assessing kidney health, monitoring disease progression, and evaluating the kidneys' efficiency in waste elimination, fluid balance, and electrolyte regulation. These tests offer critical insights into kidney function, even though routine measurements may appear normal until there is a significant decline in the glomerular filtration rate or GFR. Typically, signs of kidney impairment only become evident when the GFR falls to about 50% of its normal level.
248

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unhealthy fat distribution as a sex-specific predictor of declining hippocampus insulin sensitivity.

Diabetologia·2026
Same author

Effect of Finerenone on Albuminuria in Type 1 Diabetes by Baseline HbA1c Level and Diabetes Duration: An Exploratory Analysis of the FINE-ONE Trial.

Diabetes care·2026
Same author

Cancer prevention through metabolic remission.

Nature reviews. Endocrinology·2026
Same author

Diabetes Mellitus and the Heart.

Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association·2026
Same author

Hyperaldosteronism in Mice Lacking the Distal Polybasic Tract of the γ-Subunit of the Epithelial Sodium Channel During Sodium Restriction.

Acta physiologica (Oxford, England)·2026
Same author

Therapy of Type 2 Diabetes.

Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association·2026

Related Experiment Video

Updated: Dec 9, 2025

Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice
09:43

Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice

Published on: June 8, 2022

3.3K

Proteasuria in nephrotic syndrome-quantification and proteomic profiling.

Matthias Wörn1, Bernhard N Bohnert2, Fawza Alenazi1

  • 1Department of Internal Medicine, Division of Endocrinology, Diabetology and Nephrology, University Hospital Tübingen, Germany.

Journal of Proteomics
|September 14, 2020
PubMed
Summary

Nephrotic syndrome significantly increases urinary protease activity due to elevated excretion of active serine proteases from plasma. This "proteasuria" offers potential diagnostic markers for renal disease.

Keywords:
Nephrotic syndromeProtease activityProteasesProteasuriaProteinuria

More Related Videos

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
10:31

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

501
Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
14:48

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

Published on: May 8, 2021

7.4K

Related Experiment Videos

Last Updated: Dec 9, 2025

Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice
09:43

Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice

Published on: June 8, 2022

3.3K
Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
10:31

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

501
Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
14:48

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

Published on: May 8, 2021

7.4K

Area of Science:

  • Biochemistry
  • Nephrology
  • Proteomics

Background:

  • Nephrotic syndrome is marked by urinary proteases (proteasuria), but their quantity, activity, and identity are poorly understood.
  • Understanding these proteases is crucial for diagnosing and potentially treating kidney diseases.

Purpose of the Study:

  • To quantify protease activity and identify specific proteases in urine from healthy and nephrotic individuals and mice.
  • To investigate the role of serine proteases in nephrotic syndrome-associated proteasuria.

Main Methods:

  • Developed a fluorescence-based assay to measure total protease activity in urine.
  • Utilized specific protease inhibitors to determine protease class activity.
  • Employed tandem mass spectrometry (MS/MS) to identify individual proteases in urine samples.

Main Results:

  • Urinary protease activity was significantly higher in patients with nephrotic syndrome compared to healthy controls.
  • The increased activity was primarily attributed to active serine proteases, sensitive to inhibitors like AEBSF and aprotinin.
  • MS/MS analysis identified proteases from coagulation and complement cascades in nephrotic urine, alongside renally expressed proteases.

Conclusions:

  • Nephrotic syndrome causes increased urinary excretion of active plasma proteases (proteasuria), predominantly serine proteases.
  • These findings in humans and mice suggest a conserved pathophysiology.
  • The identified urinary proteases represent potential biomarkers for renal disease and therapeutic targets.