A near-infrared fluorogenic probe with fast response for detecting sodium dithionite in living cells

Developing a reliable fluorescence probe is crucial for accurately monitoring sodium dithionite (Na₂S₂O₄, SDT) in biosystems, but the current reported azo-based ones suffers from short excitation/emission wavelengths and relative slow response speed. To address this issue, we herein present a novel near-infrared emissive fluorescence probe for SDT, namely DCM-MQ, consisting of a dicyanomethylene-benzopyran fluorogenic reporter and a 1-methylquinolinium as recognition moiety. On the basis of the specific reduction mechanism, DCM-MQ exhibited a rapid colorimetric and fluorescent recognition for SDT (less than 3 s) with large Stokes shift (112 nm) and high sensitivity (detection limit was 19 nM). The fluorescence imaging results demonstrate that DCM-MQ is competent for monitoring SDT in living systems.