Enhanced Scenedesmus sp. growth in response to gibberellin secretion by symbiotic bacteria

Microalgae usually co-exist with bacteria, which may influence the microalgal growth, in aquatic environment. In this study, thirteen strains that can promote microalgal growth were isolated from Scenedesmus sp. LX1 culture. Additional results showed that these strains could secrete gibberellin (GA), which is a phytohormones, promoting the growth and metabolism of the Scenedesmus sp. LX1. Low concentration (0.1 mg L^-1) of GA can increase the microalgae biomass by 51% after 4 days. GA could enhance the photosynthetic activity by increasing the photosynthetic pigment content, such as culture after 2 h with low GA concentration (0.1 mg L^-1), chlorophyll a and β-carotene increased from 0.59 μg per 10⁶ cells to 0.72 μg per 10⁶ cells and from 0.20 μg per 10⁶ cells to 0.38 μg per 10⁶ cells, respectively. In addition, GA could also stimulate the dehydrogenase activity, ATP accumulation, and carbonic anhydrase activity to increase the metabolic activity of the microalgae. Interestingly, the microalgae can selectively enhance the bacterial GA secretion in turn, indicating that there was a specific feedback regulation mechanism between the microalgae and the bacteria. The results of this study show a new mechanism of symbiotic-bacteria that enhances microalgal growth. It's a great significance to understand the microalgal growth and water bloom in aquatic environment.