Long non-coding RNA MIR4300HG polymorphisms are associated with postoperative nausea and vomiting: a genome-wide...

Area of Science:

• Genetics
• Pharmacogenomics
• Molecular Biology

Background:

• Postoperative nausea and vomiting (PONV) is influenced by genetic factors, including single-nucleotide polymorphisms (SNPs).
• Previous genetic studies on PONV lack broad applicability due to population-specific genetic variations.
• A Japanese-specific DNA microarray was developed for high-throughput genotyping to address these limitations.

Purpose of the Study:

• To identify SNPs associated with PONV on a genome-wide scale in a Japanese surgical patient cohort.
• To investigate population-specific genetic variations contributing to PONV susceptibility.
• To validate candidate SNPs associated with PONV using real-time PCR.

Main Methods:

• Genome-wide association study (GWAS) using a Japanese-specific DNA microarray on 24 female patients.
• Imputation of genotypes for over 24 million SNPs.
• Selection of 4 candidate SNPs based on in silico functional annotation.
• Validation of 4 candidate SNPs in 255 patients using real-time PCR.

Main Results:

• Genome-wide analysis identified 78 SNPs associated with PONV incidence.
• The T > C variant of rs11232965 in the long non-coding RNA MIR4300HG showed a significant association with reduced PONV incidence (p = 0.01 and 0.007).

Conclusions:

• A novel SNP, rs11232965, in MIR4300HG is significantly associated with PONV.
• The rs11232965 T > C variant acts as a protective factor against PONV incidence.
• This finding contributes to understanding the genetic basis of PONV in the Japanese population.