Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

11.4K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.4K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

15.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
15.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of synthetic oxygen-generating system on cell survival under hypoxic condition in vitro.

Journal of biomaterials science. Polymer edition·2021
Same author

Automatic ECG Classification Using Continuous Wavelet Transform and Convolutional Neural Network.

Entropy (Basel, Switzerland)·2021
Same author

Field theory of reaction-diffusion: Law of mass action with an energetic variational approach.

Physical review. E·2021
Same author

The burden of liver cirrhosis and underlying etiologies: results from the global burden of disease study 2017.

Aging·2021
Same author

Cotransplantation of mesenchymal stem cells and endothelial progenitor cells for treating steroid-induced osteonecrosis of the femoral head.

Stem cells translational medicine·2021
Same author

Panax Notoginseng Saponins Prevent Bone Loss by Promoting Angiogenesis in an Osteoporotic Mouse Model.

BioMed research international·2021

Related Experiment Video

Updated: Dec 9, 2025

Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level
09:45

Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level

Published on: March 14, 2022

3.2K

Single-Cell RNA Sequencing Unveils Unique Transcriptomic Signatures of Organ-Specific Endothelial Cells.

David T Paik1,2,3, Lei Tian1,2,3, Ian M Williams1,2,3,4

  • 1Stanford Cardiovascular Institute (D.T.P., L.T., I.M.W., H.Z., C.L., R.M., S.M.W., K.R.-H., J.C.W.), Stanford University, CA.

Circulation
|September 15, 2020
PubMed
Summary

This study reveals how endothelial cell (EC) gene expression varies by tissue, identifying key genes and pathways that maintain EC identity. These findings advance our understanding of vascular biology and cell specificity.

Keywords:
computational biologyendothelial cellssequence analysis, RNA

More Related Videos

Isolation of Endothelial Cells from the Lumen of Mouse Carotid Arteries for Single-Cell Multi-Omics Experiments
09:12

Isolation of Endothelial Cells from the Lumen of Mouse Carotid Arteries for Single-Cell Multi-Omics Experiments

Published on: October 4, 2021

3.7K
RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells
18:30

RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells

Published on: February 13, 2013

22.3K

Related Experiment Videos

Last Updated: Dec 9, 2025

Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level
09:45

Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level

Published on: March 14, 2022

3.2K
Isolation of Endothelial Cells from the Lumen of Mouse Carotid Arteries for Single-Cell Multi-Omics Experiments
09:12

Isolation of Endothelial Cells from the Lumen of Mouse Carotid Arteries for Single-Cell Multi-Omics Experiments

Published on: October 4, 2021

3.7K
RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells
18:30

RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells

Published on: February 13, 2013

22.3K

Area of Science:

  • Vascular Biology
  • Transcriptomics
  • Bioinformatics

Background:

  • Endothelial cells (ECs) exhibit functional diversity across different tissues and vessels.
  • The molecular basis for this endothelial cell heterogeneity remains incompletely understood.

Purpose of the Study:

  • To investigate the transcriptomic underpinnings of endothelial cell specificity.
  • To identify molecular networks maintaining tissue-specific endothelial cell identity.

Main Methods:

  • Analysis of single-cell RNA sequencing data from the Tabula Muris consortium.
  • Application of bioinformatics tools for uncovering markers and sources of heterogeneity.
  • Graph-based clustering to analyze transcriptomic relationships between tissue-specific ECs.

Main Results:

  • Validated single-cell RNA sequencing (scRNA-seq) with bulk RNA sequencing for tissue-specific EC transcriptomes.
  • Identified distinct clustering patterns for ECs based on tissue origin, with some overlap observed.
  • Discovered novel tissue-specific EC markers, signaling pathways, and sex-specific gene expression (e.g., Lars2 in male ECs).
  • Confirmed conservation of mouse heart and lung EC markers in human fetal tissues.
  • Uncovered potential angiocrine interactions through ligand-receptor expression analysis.

Conclusions:

  • Single-cell RNA sequencing data from Tabula Muris effectively elucidated transcriptional networks driving EC identity.
  • Identified novel angiocrine and functional relationships involving tissue-specific ECs.