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Updated: Dec 9, 2025
Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
Published on: March 16, 2017
A sensitive and selective fluorescent probe for the detection of endogenous peroxynitrite (ONOO-) in living cells
Yaru Sun1, Baoli Dong1, Yaru Lu1
1Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, University of Jinan, Jinan, Shandong 250022, People's Republic of China. weiyinglin2013@163.com.
Abstract:
Peroxynitrite (ONOO-) is one of reactive oxygen species, and plays a vital role in numeorus physiological and pathological processes. Given that the ONOO- level is closely related with various serious diseases, the in situ and real time detection of endogenous ONOO- is highly important for the in-depth study of its roles in living systems. Herein, we present a new fluorescent probe (RHPN) for the real-time detection of intracellular ONOO-. The probe RHPN consists of a rhodamine analogue and an arylhydrazide group as a response site for ONOO-. In response to ONOO-, the probe RHPN converts to an open-ring form and generates strong fluorescence. Moreover, the probe RHPN was successfully used for the imaging of the endogenous and exogenous ONOO- level changes in living cells.
