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Updated: Dec 9, 2025

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells
Published on: July 22, 2013
Mitochondrial-targeted deep-red fluorescent probe for ATP and its application in living cells and zebrafish
Wentao Peng1, Mingxing Hu2, Jian Zhang3
1Nursing Department of the West China Second University Hospital, Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, Sichuan University, Chengdu, Sichuan Province 610041, P. R. China.
Abstract:
A novel mitochondrial-targeted deep-red fluorescence ATP probe, NIR-A, is reported. The probe showed a fast, selective, and reversible response for ATP with a significant turn-on fluorescence signal at 663 nm with a large Stokes shift of 81 nm. Additionally, the introduction of TPP enabled TPP-endowed NIR-A to be enriched predominantly in the mitochondria. NIR-A was successfully applied to monitor ATP fluctuation in Ramos cells and zebrafish in real-time with good biocompatibility.

