Related Experiment Video
Updated: Dec 9, 2025

Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method
Published on: July 27, 2016
Preparative purification of corilagin from Phyllanthus by combining ionic liquid extraction, prep-HPLC, and
Xiaodong Hou1, Zitao Cheng1, Jiao Wang1
1Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, Shandong 266101, China. wangj362@nenu.edu.cn.
Abstract:
In this study, a green extraction and purification process for the rapid preparation of corilagin from Phyllanthus has been designed using an aqueous ionic liquid coupled with preparative high-performance liquid chromatography (prep-HPLC) and precipitation. The results showed that the optimum extraction process for corilagin involved mixing Phyllanthus tenellus Roxb. with 0.4 M [BMIm]Br at a liquid-solid ratio of 10 : 1 and dispersing the mixture by ultrasonication at 50 °C for 15 min. Macroporous resin D101 and prep-HPLC were employed for [BMIm]Br removal and corilagin separation to yield corilagin of 86.49% purity. Subsequently, corilagin was further purified by water precipitation to achieve 99.12% purity. The results indicated the successful development of a new rapid and green process to prepare corilagin on a large scale from plants using [BMIm]Br. This promising process can be applied for the preparative separation and purification of other active compounds from complex plant systems.
Related Concept Videos
Precipitation and Co-precipitation
Extraction: Advanced Methods
Colloidal precipitates
High-Performance Liquid Chromatography: Elution Process
Coagulation
Types Of Column Chromatography
Gel Filtration Chromatography
When the...

