Related Experiment Video
Updated: Dec 9, 2025

Scanning-probe Single-electron Capacitance Spectroscopy
Published on: July 30, 2013
A de-waxing methodology for scanning probe microscopy
Safaa Al Jedani1, Caroline I Smith1, Philip Gunning2
1Department of Physics, University of Liverpool, L69 7ZE, UK. peterw@liverpool.ac.uk.
Abstract:
A de-waxing protocol that successfully removes paraffin from tissue microarray (TMA) cores of fixed tissue obtained from oral cancer is described. The success of the protocol is demonstrated by the comparison of Fourier transform infrared (FTIR) results obtained on paraffin-embedded and de-waxed tissue and the absence of any significant correlations between infrared scanning near-field optical microscopy (SNOM) images of de-waxed tissue obtained at the three main paraffin IR peaks. The success of the protocol in removing paraffin from tissue is also demonstrated by images obtained with scanning electron microscopy (SEM) and by energy dispersive spectra (EDS) of a de-waxed CaF2 disc which shows no significant contribution from carbon. The FTIR spectra of the de-waxed TMA core overlaps that obtained from OE19 oesophageal cancer cells which had never been exposed to paraffin.
More Related Videos
13:15Quantitative and Qualitative Examination of Particle-particle Interactions Using Colloidal Probe Nanoscopy
Published on: July 18, 2014
09:12Functionalization of Single-walled Carbon Nanotubes with Thermo-reversible Block Copolymers and Characterization by Small-angle Neutron Scattering
Published on: June 1, 2016
Related Concept Videos
Overview of Microscopy Techniques
Atomic Force Microscopy
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
Preparation of Samples for Electron Microscopy