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Updated: Dec 9, 2025

An Innovative Method for Exosome Quantification and Size Measurement
Published on: January 17, 2015
TIRF Microscopy as a Tool to Determine Exosome Composition
Noa B Martín-Cófreces1,2,3, Daniel Torralba4,5, Marta Lozano-Prieto4,5
1Servicio de Inmunología. Hospital Universitario de la Princesa, Universidad Autónoma de Madrid, Instituto Investigación Sanitaria Princesa (IIS-IP), Madrid, Spain. noa.martin@salud.madrid.org.
This study presents a new microscopy method to analyze the contents of individual extracellular vesicles (EVs). This technique allows for better visualization and selection of EVs with specific biological functions.
Area of Science:
- Biochemistry
- Cell Biology
- Microscopy
Background:
- Exosomes, a type of extracellular vesicle (EV), carry biomolecules like proteins and nucleic acids.
- EVs are crucial for intercellular communication in immunity and disease, including cancer.
- Accurate assessment of EV content is vital for selecting vesicles with specific biological activities.
Purpose of the Study:
- To develop a user-friendly protocol for determining the content of individual EVs.
- To enable visualization of EV contents, which are often obscured due to their small size.
Main Methods:
- Utilizing Total Internal Reflection Fluorescence (TIRF) microscopy.
- Implementing a protocol for microscopy imaging of individual EVs.
- Employing user-friendly analysis of microscopy data.
Main Results:
- Demonstration of a protocol for analyzing individual EV content.
- Improved visualization of biomolecules within EVs.
- Facilitation of the selection of specific EVs based on their content.
Conclusions:
- The developed protocol enables precise assessment of individual EV content.
- TIRF microscopy offers a valuable tool for studying EV composition.
- This method aids in identifying and selecting EVs for specialized biological applications.
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