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Related Experiment Videos

Do osmotic forces play a role in renin secretion?

O Skøtt1

  • 1Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor 48109.

The American Journal of Physiology
|July 1, 1988
PubMed
Summary

Secretory granule swelling precedes exocytosis in renin release. Granular proton gradients may drive swelling, influencing macula densa-mediated renin secretion.

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Area of Science:

  • Cell Biology
  • Endocrinology
  • Renal Physiology

Background:

  • Exocytosis involves secretory granule swelling.
  • Granular proton gradients are hypothesized to mediate preexocytic swelling.
  • Renin release from juxtaglomerular cells is sensitive to extracellular osmolality.

Purpose of the Study:

  • To investigate the role of secretory granule swelling in renin exocytosis.
  • To determine the contribution of granular proton gradients to preexocytic swelling.
  • To explore the osmosensitivity of renin release and its potential role in macula densa function.

Main Methods:

  • Studied exocytosis of renin from isolated juxtaglomerular cells.
  • Manipulated extracellular osmolality to induce granule swelling.
  • Assessed the effects of proton gradient dissipation on renin release and osmosensitivity.

Main Results:

  • Extracellular hyposmolality induced secretory granule swelling, plasma membrane fusion, and increased renin release.
  • Granule swelling stimulated renin release even at constant extracellular osmolality.
  • Dissipating granular proton gradients inhibited renin release but not osmosensitivity.

Conclusions:

  • Secretory granule swelling precedes renin exocytosis.
  • Granular proton gradients may contribute to preexocytic swelling under constant osmolality.
  • Osmosensitivity of renin release is potentially crucial for macula densa-mediated regulation.

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