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Long intergenic non-protein coding RNA-467 targets microRNA-451a in human colorectal cancer
Yang Bai1,2, Haiyan Wu1, Bin Han1
1Department of Pharmacology, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
Oncology Letters
|September 16, 2020
Summary
Long non-coding RNA (lncRNA) linc00467 is overexpressed in colorectal cancer (CRC), promoting metastasis. It targets microRNA-451a, increasing proliferation and inhibiting apoptosis in CRC cells.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cancer.
- Overexpression of lncRNAs is common in colorectal cancer (CRC).
- Predictive signatures for CRC metastasis based on lncRNAs are limited.
Purpose of the Study:
- Identify key lncRNAs involved in human CRC metastasis.
- Determine the downstream targets of these lncRNAs.
- Establish a potential lncRNA signature for CRC metastasis prediction.
Main Methods:
- Analysis of 31 human CRC biopsy samples.
- Evaluation of long intergenic non-protein coding RNA-467 (linc00467) expression.
- Cellular proliferation and apoptosis assays in linc00467-knockout CRC cells (HT29, HCT116).
- Bioinformatics analysis to predict downstream targets.
Main Results:
- linc00467 was significantly overexpressed in CRC tissues.
- linc00467 expression correlated with metastasis and Tumor-Node-Metastasis stage.
- Knockout of linc00467 reduced proliferation and increased apoptosis in CRC cells (P<0.05).
- Bioinformatics analysis identified microRNA (miR)-451a as a downstream target of linc00467.
Conclusions:
- Increased linc00467 expression promotes CRC metastasis.
- linc00467 targets miR-451a to enhance CRC carcinogenesis and metastasis.
- This interaction leads to increased cellular proliferation and decreased apoptosis in human CRC cells.
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