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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Related Experiment Video

Updated: Dec 8, 2025

Locked Nucleic Acid Flow Cytometry-fluorescence in situ Hybridization LNA flow-FISH: a Method for Bacterial Small RNA Detection

Locked Nucleic Acid Flow Cytometry-fluorescence in situ Hybridization LNA flow-FISH: a Method for Bacterial Small RNA Detection

Published on: January 10, 2012

Single Cell RNA Expression Analysis Using Flow Cytometry Based on Specific Probe Ligation and Rolling Circle

Yansong Zhao¹, Chen Lin¹, Pengcheng Wu¹

¹School of Biomedical Sciences and School of Medicine, Huaqiao University, Quanzhou 362021, China.

|September 16, 2020

Summary

This summary is machine-generated.

A new method, RCA-Flow, enables high-throughput single-cell RNA analysis, detecting mRNAs, microRNAs, and circular RNAs. This versatile technique overcomes antibody limitations for broader biomedical and clinical applications.

Keywords:

RNA expression circular RNA flow cytometry microRNA rolling circle amplification single cell

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Last Updated: Dec 8, 2025

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Published on: April 11, 2015

Area of Science:

Molecular Biology
Biotechnology
Genomics

Background:

Flow cytometry is crucial for high-throughput single-cell analysis.
Current methods often rely on antibody staining, which is limited by antibody availability and quality.
Analyzing diverse RNA types like microRNAs and circular RNAs via flow cytometry is challenging.

Purpose of the Study:

To develop a novel flow cytometry technique for comprehensive single-cell RNA expression analysis.
To overcome the limitations of antibody-dependent methods in flow cytometry.
To enable the detection of various RNA species, including mRNAs, microRNAs, and circular RNAs.

Main Methods:

Development of a novel flow cytometry RNA detection technique named RCA-Flow.
Application of RCA-Flow for analyzing messenger RNAs (mRNAs).
Utilizing RCA-Flow for the detection of microRNAs (miRNAs) and circular RNAs (circRNAs).

Main Results:

RCA-Flow successfully performs high-throughput single-cell RNA expression analysis.
The method demonstrates capability in detecting mRNAs, which are standard targets.
Crucially, RCA-Flow can also analyze microRNAs and circular RNAs, which are typically difficult to detect with existing flow cytometry approaches.

Conclusions:

RCA-Flow offers a versatile and powerful tool for single-cell RNA analysis.
The technique expands the scope of detectable RNA molecules beyond conventional methods.
RCA-Flow holds significant potential for advancing biomedical research and clinical diagnostics.